C1q Binding Assays
BioOutsource offers off-the-shelf C1q binding assays to support monoclonal antibody manufacture, process development, characterization and similarity studies. Our C1q binding assay method can be qualified specifically for your product.
The complement system complements the ability of antibodies to clear pathogens and cancerous cells from the body. It is a collection of small proteins present in the blood that typically circulate as inactive precursors. The classical pathway is triggered by activation of the C1-complex which is composed of a single C1q molecule with 2 molecules of C1r and C1s, and occurs when C1q binds multiple IgG molecules complexed with antigen; this can lead to a signalling cascade culminating in complement-dependent cytotoxicity (CDC). Activation of complement is experimentally determined using the C1q binding assay.