Frequently Asked Questions
The Cellca CHO host cell line used for all client projects is the CHO DG44 cell line directly obtained by its inventor, Dr. Lawrence Chasin, US. Cellca scientists have adapted this cell line to suspension cell growth in animal component free, chemically defined media, and it is, therefore, GMP compliant. All lots of serum utilized in the initial development phase of the cell line, to the point where it was adapted to chemically defined cell culture medium, have been fully documented, including source information and all certificates of analysis. Cellca has the full cell line history for the parental CHO DG44 cell line. All current Cellca cell line development programs are initiated from the working cell bank (WCB) of the parental host. A full biological safety testing package for this WCB is available as part of the cell line history.
Are there any industry concerns about using the DHFR/MTXR expression platform to make stable cell lines?
Genetic stability of CHO cells, in general, is a potential concern. Thus, in Cellca’s normal cell line development program a stability study is integrated to prove the stability of created cell lines. Around 90% of all clones generated using the Cellca platform are stable.
Cellca utilizes the CASY, (Omni Life Science) or the Vi-Cell (Beckman Coulter) for cell counts and viability measurements.
Cellca measures protein titer using an Octet instrument by Forte Bio (Pall) or Protein A HPLC.