Improved Clone Evaluation and Selection of CHO Cell Lines using ambr15®

8th August 2017

Category: Cell Line Development

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By: Anton Stefan - Scientist, Dirk Mueller - Team Leader, Dr Christoph Zehe - Head of Technology Development, Michael Grauf - Scientist,

The global market for biopharmaceutical products continues to expand, making it a highly attractive market for biopharmaceutical companies. Due to the rising number of projects with variant protein drug candidate molecules, increasing productivity of cell culture process development to support material production for early phase clinical trials has been an important task in recent years. More

Cell Line Development (CLD) 2.0

6th July 2017

Category: Cell Line Development

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By: Caroline Hauser - Team Leader, Dr Christoph Zehe - Head of Technology Development, Juliana Schubert - Scientist, Kristin Thiele - Scientist,

The production of biologics in Chinese Hamster Ovary cells (CHO) is becoming more and more challenging due to an increasing number of complex and difficult-to-express (DTE) molecules. Nevertheless, general Cell Line Development (CLD) platform processes should provide reliable high-titer cell lines irrespective of the therapeutic protein formats to be expressed. In the last 20 years More

How to achieve high-yielding cell lines

30th May 2017

Category: Cell Line Development

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By: Caroline Hauser - Team Leader, Dr Christoph Zehe - Head of Technology Development, Juliana Schubert - Scientist, Nico Erb - Scientist,

The global market for biological products is expected to reach $287bn by the end of 2020. There are many drivers for the continued investment and development of therapeutic protein products, such as: an increase in incidences of cancer and autoimmune diseases, reduction in side effects, advances in technology and patent expiry of blockbuster drugs. Although More

What is the best way to approach biosimilar development?

27th March 2017

Category: Bioanalytical / Biosimilars

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By: Dr Terry Gray, Field Marketing Manager,

Part one Monoclonal antibodies have a highly specific targeted action which allows a reduction in side effects compared to small molecule drugs, which has seen them becoming a mainstay of modern medicine. The innate complexity of these molecules allows them to be potent drugs that activate molecular cascades within the immune system. So what does More

Piecing together the biosimilar puzzle: Physicochemical & Functional Analytics

Category: Bioanalytical / Biosimilars

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By: Dr Terry Gray, Field Marketing Manager,

Part two A fundamental feature of a mAb is its biological structure as this defines the functional output of the biologic, as illustrated by figure 2. Figure 2 – The interplay between the structure of a monoclonal antibody, the affinity for interaction and the resulting functional activity. Fundamentally, the sequence of a biosimilar must match More

Choosing the right assay at the right time for biosimilar development

Category: Bioanalytical / Biosimilars

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By: Dr Terry Gray, Field Marketing Manager,

Part Three As highlighted in our previous blog articles, cost & throughput are important contributing factors to consider when developing a biosimilar. Another factor to consider is the choice of assay that will be used at each stage of the biosimilar product life cycle. There are two competing areas which drive the decision making process More

Preparing for future bioprocessing challenges with the Cellca CHO Expression Platform

13th July 2016

Category: Cell Line Development

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By: Dr. Brian Wendelburg, Sr. Field Marketing Manager,

Therapeutic protein development and production is a very technically challenging, time consuming and expensive endeavor. What are some of the challenges? There are numerous critical decisions that must be made with regard to mammalian cell line choice (CHO, NS0, etc.), appropriate gene and marker expression systems, cell culture medium and feed optimization, clone selection and More

ELISA – Conception and Applications

19th August 2015

Category: Bioanalytical / Biosimilars

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By: Laura Munro, Technical Services Scientist,

Since its conception in the early 1970’s the Enzyme Linked Immunosorbent Assay has been a primary method of analyte detection. In its four decades it has become a fundamental tool in a wide range of scientific fields, its diverse nature demonstrated by its range of uses. The ELISA was the first screening test widely used More

An Orthogonal Approach to ADCC Biosimilar Testing

Category: Biosimilars

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By: Debbie Allan PhD, Technical Services Scientist,

Antibody-Dependent Cellular Cytotoxicity (ADCC) is a mechanism adopted by the immune system to remove infected or cancerous cells. Antibodies bind a specific antigen on the target cell surface which in turn binds the FcγRIIIa receptor on effector cells (e.g. Natural Killer (NK) cells) via the Fc region resulting in the lysis of the target cell. More

The Importance of Accurate Determination of ADCC Activity During Biosimilar Development

Category: Biosimilars

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By: Stefan Termen, Technical Services Scientist,

Cells infected with enveloped viruses will express viral proteins on their cell membranes, and many cancer cells will express specific surface antigens. These proteins are recognized by antibodies of the humoral immune system as targets for Antibody-Dependent Cell-mediated Cytotoxicity (ADCC). In this process, effector cells of the immune system recognize the bound antibodies through their More

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