Qualifying & Developing VEGF Binding Assay using an iQue® Screener PLUS
25th May 2018
Category: Bioanalytical
By: Ben Tyrrell, PhD - Scientist, Product Development, Katie Chapple - Scientist,
The iQue® Screener PLUS, by Intellicyt (part of the Sartorius Group), is used at Sartorius Stedim BioOutsource to achieve a faster assay throughput and deliver an air-gap-deliminated flow of samples to the detectors. Bevacizumab is a humanized monoclonal antibody (mAb) which mediates its activity through a high affinity binding to VEGF (vascular endothelial growth factor). More
Robust & Reproducible ADCC with Single Donor KILR CD16 Effector Cells
23rd May 2018
Category: Bioanalytical
By: Laura McAleer, Technical Services Scientist, Lisa Blackwood, PhD - Senior Scientist, Product Development,
The success of ADCC (antibody-dependent cell-mediated cytotoxicity) depends on the quality of the effector cells used. Traditional ADCC assays can be complex and require freshly isolated primary human cells, such as PBMC (peripheral blood mononuclear cell) or NK (natural killer) cells. However these can have inter-individual variability. While NK cell lines engineered to overexpress CD16 are More
The Path to Protein Concentration Measurement
8th May 2018
Category: Bioanalytical
Tags: Analytical Testing, Physicochemical Analysis, protein characterization
By: Stuart Wright - Scientist,
Protein Concentration Analysis Quick and accurate measurement of protein concentration is very important within the biopharmaceutical industry. This must be carried out on a large number of samples prior to performing the various physicochemical analysis involved in protein characterization or protein structural analysis, since many assays depend on knowledge of the biotherapeutic protein concentration. However, More