Cell Based Binding Assays

Evaluation of binding of monoclonal antibodies to receptors or targets on cellular membranes can provide additional information above that which can be gained from methods that utilize recombinant versions of the target. Glycosylation patterns and conformational changes may occur within different cell types to elicit different binding patterns and kinetics that may provide valuable information on your product. BioOutsource provides contract testing services to evaluate binding activity using monolayers, fixed or unfixed, with detection using traditional ELISA endpoints, electrochemiluminescence (ECL) via the mesoscale discovery (MSD) or by measuring binding of cells with the Biacore T200 using full kinetic analysis.