Molecular Weight Analysis

The determination of molecular weight is essential for preliminary assurance that the recombinant product is synthesized as expected. Molecular weight measurement can indicate potential sequence variation and provide a high level assessment of post-translational modification, including glycosylation. Our high-resolution LC-MS methods provide accurate molecular weight information and complements our size exclusion chromatographic methods.

Intact Mass Analysis and mAb Subunit Analysis by LC/MS

Our dedicated and experienced team have methods to assess the mass of the intact protein, deglycosylated protein and reduced protein.

Intact mass

Intact mass analysis is the process of determining the molecular weight of a protein. Comparison of the observed mass to the mass expected from the primary sequence provides a preliminary assurance of amino acid composition. Intact mass analysis also provides information on the nature of the main protein glycoforms, as well as other major modifications.

Deglycosylated

Deglycosylation of an IgG prior to analysis by LC/MS removes the heterogeneity associated with N-glycosylation. This is employed to assess the mass of the protein backbone without the glycans attached. Together with the intact mass data, this method provides indirect validation of the primary structure.

Reducing conditions

LC/MS analysis of the reduced monoclonal antibody is used to confirm the molecular weight of the individual light and heavy chains. In addition to confirming the data for the intact and deglycosylated mass, the analysis of smaller protein fragments means that the method is more sensitive to less abundant modifications.

Contact our scientists to discuss your project today.

Contact us

 

Sign-up to our newsletter to be the first to hear about our new services & offers

Sign-up to our newsletter to be the first to hear about our new services & offers

Enter your email address to subscribe to our newsletter which provides information about our services. By subscribing, you are agreeing to the processing of your personal information for this purpose as set out in our privacy policy