Promoter Evaluation in the Cellca CHO DG44 Expression System

4th December 2017

Category: Cell Line Development

Tags: ,

By: Ann-Catherin Leroux - Scientist, Dr Christoph Zehe - Lead Scientist,

One key component of the powerful Cellca Expression Platform is the expression vector. We have carefully selected its genetic elements to allow:

As a next step in enhancing our platform, we have evaluated several viral and mammalia promoters with regards to their impact on antibody titers and productivity.

Cellca CHO Expression Vector

First, we designed eight vector variants, each carrying a different promoter. We then transfected our robust CHO DG44 host and screened the resulting pools for titers in batch experiments. Out of the eight variants, the four promoters yielding the highest titers on pool level were chosen for fed-batch experiments. The single promoter allowing highest titer and productivity was selected for expression of four model antibodies. Compared to the previously used promoter, titers were 46 to 212% higher and the final clones showed excellent stability. Hence, we were able to identify a promoter which will allow up to threefold higher titers.

Download our scientific poster to find out more about this promoter.

 

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