Webinar – The Next Level: Optimizations of a Platform for CHO Cell Line Generation
Watch our webinar on demand now. Dr. Cornelia Lindner, Sartorius Stedim Cellca, discusses the process of optimizing a platform for CHO cell line generation.
Cornelia Lindner, Team Lead Operations, Sartorius Stedim Cellca
The market for therapeutic proteins is steadily growing and biological formats are getting more complex, resulting in a high demand for fast, reliable and efficient cell line and bioprocess development. This talk demonstrates how a CHO expression platform is improved in order to tackle and master these challenges. The presented developments comprise:
(i) Optimized expression vectors resulting in improved metabolic selection in the absence of selective agents (e.g. MTX, antibiotics), increased productivities and shortened timelines
(ii) Implementation of modern down-scale models (e.g. ambr 15 & ambr 250) for clone selection, process characterization and optimization
(iii) Process intensification leading to increased volumetric productivities and product titers > 8 g/L.
Click here to watch the webinar