Robust & Reproducible ADCC with Single Donor KILR CD16 Effector Cells
23rd May 2018
By: Laura McAleer, Technical Services Scientist, Lisa Blackwood - Scientist,
The success of ADCC (antibody-dependent cell-mediated cytotoxicity) depends on the quality of the effector cells used.
Traditional ADCC assays can be complex and require freshly isolated primary human cells, such as PBMC (peripheral blood mononuclear cell) or NK (natural killer) cells. However these can have inter-individual variability. While NK cell lines engineered to overexpress CD16 are available, they often show high background lysis in susceptible cell models in addition to high functional variability under different culture conditions.
Our study presents data on cytotoxicity assays using single donor-derived, engineered effector cells which stably express CD16, the KILR® CD16 Effector Cells.
The data from our study shows that our optimized cells reduce long term variability, have excellent repeatability and precision, demonstrate excellent accuracy and linearity, and are also compatible with the T cell redirection with the clinical molecule blinatumomab.
Download our scientific poster to find out more about the methods and data for driving a robust and reproducible ADCC & T cell redirection with single donor KILR® CD16 Effector Cells.